A simple precise and accurate RP-HPLC method has been developed for estimation of ornidazole and miconazole in tablet dosage form. Ornidazole and miconazole chromatographic separation was carried out on Hiber Lichrospher® 100, RP-18e (5 µm), Merck Ltd. India, 250 mm L × 4.6 mm I.D in size, using mobile phase Acetonitrile: methanol (80:20 % v/v) and detected at 219 nm. Validation of developed method was performed according to ICH Q2 R1guidline. The linearity concentration range was 20 µg/ml -60 µg/ml for ORN and 4 µg/ml-12 µg/ml for MIC. The retention times of ornidazole and miconazole were found to be 3.53 min and 7.41 min respectively. The limit of quantification (LOQ) for ornidazole and miconazole were found to be 4.894 µg/ml and 0.898 µg/ml respectively. Then the limit of detection (LOD) for ornidazole and miconazole were found to be 1.615 µg/ml and 0.57 µg/ml. The percentage recoveries obtained for ornidazole and miconazole ranges from 98.36% - 101.52% and 98.88% - 101.50% respectively. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate which is useful for the routine determination of ornidazole and miconazole in its tablet dosage form.
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